Document Type : Original Article

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Department of Biotechnology, Faculty of Science, Turkmen State University, Ishqabad, Turkmenistan

Received: 16 January 2021                    Accepted: 03 February 2021                    Published: 01 March 2021

 10.22034/MBT.2021.122555                                                         XML Files


A new two-step route for the preparation of nano-scaled magnetic chitosan particles has been developed, different from reported one-step in situ preparation and two-step preparation method of reversedphase suspension, Triton X-100 reversed-phase water-in-oil microemulsion encapsulation method was employed in coating the pre-prepared Fe3O4 nanoparticles with chitosan. Two model anticancer drugs, Doxorubicin (DOX) and Methotrexate (MTX) were loaded effectively to functionalized MNPs through electrostatic interactions. Our developed UV method was applied for simultaneous determination of DOX and MTX. It was concluded that our findings may open the possibilities for pH-responsive targeted delivery of DOX and MTX to the cancerous tissues.