Couroupita guianensis Aubl. commonly is the scientific name of cannon ball tree, which is belongs to Lecythidaceae family and is widely used in Chinese traditional medicine. This tree is in danger dau to vast destruction in their habitat almost in its native range because of human settlement and other developmental-related activities. Native populations of C. guianensis were collected from different parts of the Indian states. To study the genetic diversity of C. guianensis population we performed RAPD – PCR procedure with several primers.